Sets of trisubstituted thiazoles (4a-4i) were synthesized by reaction of substituted N-(piperidinyl/ piperazinyl/N-methylpiperazinyl-4-thioyl) benzamide (3) with 3-(bromoacetyl) coumarin, respectively. The (3) were synthesized by nucleophilic addition of benzoyl isothiocyanate/ substituted benzoyl isothiocyanate and piperidine/ piperazine/4-methylpiperazine in equimolar quantity at reflux temperature. The synthesized compounds (4a-4i) were screened for their in vitro antibacterial activity against S. aureus, B. subtilis and E. coli using ciprofloxacin as standard drug. The targeted compounds (4a-4i) were evaluated for their in vivo anti-inflammatory activity in carrageenin-induced rat hind paw oedema model at three graded doses employed at 10, 20 and 40 mg/kg body weight using ibuprofen standard drug. Compounds (4a-4i) were also evaluated for ADP-induced platelet aggregation of platelet-rich plasma aspirin was used as positive control. Among all the targeted compounds 4h and 4i showed maximum antibacterial activity against all the three bacteria, while 4b showed maximum 89.6% antiplatelet activity. Compound 4c showed maximum anti-inflammatory activity of 33% protection at 10 mg/kg and 57% protection at 20 mg/kg to inflamed paw.
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